ENTER PROTEIN SEQUENCE

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Amino Acid 3 letter code 1 letter code
Alanine Ala A
Cysteine Cys C
Aspartic acid Asp D
Glutamic acid Glu E
Phenylalanine Phe F
Glycine Gly G
Histidine His H
Isoleucine Ile I
Lysine Lys K
Leucine Lys K
Lysine Leu L
Methionine Met M
Asparagine Asn N
Proline Pro P
Glutamine Gln Q
Serine Ser S
Threonine Thr T
Valine Val V
Tryptophan Trp W
Tyrosine Tyr Y
  • Overlapping Peptide Library
    Overlap Library

    The length of the input sequence should be greater than 10.

    Set Overlapping Peptides Design Parameters:

    Overlapping Peptide Library

    Overlapping Peptide Library can be used in epitope mapping. The Peptide library generation process is defined by two parameters, peptide length and offset number. The offset number reflects the degree of overlapping. Careful selections of these two parameters are important to achieve optimum balance between data value and the experiment cost. As a general guideline, shorter peptides are easier to synthesize but they have less chances for multiple epitope hits. Greater degree of overlapping (small offset number) gives more chances of multiple epitope hits. Both above stated cases increase the number of peptides to be synthesized for the peptide library. Read More »

  • Alanine Scanning Library
    alanine scan Library

    The length of the input sequence should be between 6 and 200 inclusively.

    Alanine Scanning Library

    Alanine, the smallest chiral natural amino acid is used to substitute non-alanine residues at each position of the original peptide. The Alanine Scanning Library can be used to determine the relative importance of each substituted residues toward the original peptide's functionality since knocked off key residues reflect diminished peptide activity. Read More »

  • Truncation Library
    alanine scan Library

    The length of the input sequence should be between 10 and 200 inclusively.

    Truncation Library

    Truncation Library can be used to identify the shortest amino acid sequence needed for the peptide activity. The truncation process is carried out via a systemic reduction of resides from each flank of the original peptide. With the knowledge of the positions of key residues elucidated through Alanine Scanning Library, the construction of the truncation library could also be centered around these key amino acid residues. Read More »

  • Positional Scanning Library
    position scan Library

    The length of the input sequence should be between 10 and 200 inclusively.

    (please use ';' between adjacent position input)

    Positional Scanning Library

    Positional Scanning Library is one of the key methods for sequence optimization. Achieved by substituting selected amino acid residues by all other natural amino acids one at a time, it has the capability to locate potential more favorable residue(s) at specified position(s) for enhanced peptide activity. Read More »

  • Random Library
    Random Library

    The length of the input sequence should be between 10 and 200 inclusively.

    (The number of positions N is between 2-3 ,
    The number of random combinations for N=2 is 400,
    and that for N=3 is 8000+400+400+400=9200)
    [ please use ';' between adjacent position input ],

    Random library

    Used in sequence optimization, Random Library is similar to positional scanning library. It's constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity. Read More »

  • Scrambled Library
    Scrambled Library

    The length of the input sequence should be between 10 and 200 inclusively.

    Scrambled Library

    Scramble Library is constructed by carrying out permutation on the original peptide's sequence. It has the potential to give all possible alternatives and offers and represents the highest degree of variability for peptide library. Read More »

  • T Cell Truncated Library
    T-cell truncated library

    Input the peptide length

    Note: for most T cell epitope mapping applications, choose 11-mers as starting points for your peptide pools. The design tool will automatically generate 11-, 10-, 9- and 8-mer peptides. For other peptide lengths (ex. n-mer), n-, n-1, n-2 and n-3-mers will be generated.

    T-cell truncated library:

    T cell epitope identification is a critical part of designing highly specific immunotherapies. The T cell truncated library spans sequentially truncated sequences across a peptide sequence to identify important epitopes. Once identified, these sequences can be used to guide the development of targeted drugs or engineer specialized T cells for T cell therapy. Read More »

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