Quote/Order Request Now

Home » Gene Synthesis & Molecular Biology Services » Plasmid DNA Preparation » Plasmid Linearization Service | GenScript

Overview

GenScript’s gene to mRNA end-to-end solution offers high quality optimized IVT templates to deliver high integrity mRNA for exceptional protein expression. Our plasmid linearization service offers plasmid linearization for mRNA production with restriction enzyme after adding a PolyA tail, containing T7 promoter, 5' & 3' UTR, and PolyA Tail.

Molecular Biology

One-Stop Solution for ALL Your mRNA Needs

gene synthesis icon
Gene Synthesis
plasmid preparation icon
Plasmid Preparation
linearization icon
Linearization
mrna synthesis icon
mRNA Synthesis
Request Now

Service Description

Pre-clinical Grade Research Grade Industrial Grade
Service packages Linearization (Preclinical) Linearization (Research/Industrial)
Price Starting from $480 Starting from $89
Quantity >5 mg >100 µg
QC
Endotoxin 10EU/mg (default) 10EU/mg (industrial) or no control
(Research)
Linearization purification AKTA (Circular plasmid) + Anion exchange chromatography
(Linearization plasmid) 		Default: Anion exchange chromatography
(Circular plasmid) + Isopropanol
precipitation (Linearization plasmid)
Linearization QC HPLC ≥ 95% (need 20 µg needed for HPLC) AGE (load 200 ng, see single band)
Host cell DNA <5%, qPCR AGE,<15% plasmid band
Total protein Nano orange <2% (default) N/A
RNA Non-detectable at 200 ng by AGE Non-detectable at 200ng by AGE

Extensive list of QC add-ons to promise highest quality input to deliver superior mRNA yield

Add-on Linearization (Preclinical) Linearization (Research/Industrial)
Additional Linearization purification N/A Optional--Magnetic bead purification (<100µg) or Anion exchange chromatography（>100 µg）
Low total protein NanoOrange <1%, (additional cost) only when quantity>5 mg NanoOrange <2% only when quantity>5 mg
Advanced Endotoxin Removal <0.005EU /µg quantitative TAL (default), LAL (optional) <0.005EU /µg quantitative TAL (default), LAL (optional)
Mycoplasma Contamination Assay Luminescence detection kit N/A
Detection of Kanamycin ELISA N/A
Quantitative Linearization by HPLC Additional cost if >98% Tentative >95%,  Additional cost if >98%
Animal Free Production Process with TSE/BSE certification RNase, BsaI/EcoRI/BspQI , TSE/BSE certification RNase, BsaI/EcoRI/BspQI , TSE/BSE certification
Bio-burden Assay 48 hr YPD and LB 48 hr YPD and LB
Nicked plasmid treatment T4 ligase treated circularization T4 ligase treated circularization
Request Now

Better Input Better Outcome

plasmid glp icon

Pure linearized plasmid
lead to successful IVT

plasmid glp icon

Higher mRNA yield,
purity and better protein
expression

plasmid glp icon

Strict endotoxin control and less
safety concerns to your mRNA
and downstream application

plasmid glp icon

Strict control of RNA, host
DNA, residual host protein
or enzymes lead to less
noise from contaminants

Learn More

Pure linearized plasmid lead to successful IVT

Linearized plasmid purity% Linear plasmid% IVT yield (μg)
50% 50% 16.8
90% 90% 18.4
95% 95% 24.7
98% 98% 23.8
>98% 100% 24.9
Request Now

Get in Touch
with GenScript Plasmid Linearization Service

Get in Touch with GenScript Plasmid Linearization Service
Please enter a valid first name.
Please enter a valid last name.
Please enter a valid email name.
Please select a valid industry.
Please select a valid country.
Please enter a valid Zipcode.
Please enter a valid institution/company.
Please enter a message in the textarea.

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*
Skip