Imagine finding a happy medium between random and rational protein engineering; this sums up “Precision Mutant Libraries.” By taking advantage of Next-Generation semiconductor-based DNA Synthesis Technology operating within an automated platform, you can introduce exact nucleotide changes, allowing testing your predictions and unraveling connections between your protein’s sequence, structure, and function.
Vesna Mitchell, Ph.D.
Codexis Protein Engineering Experts
Reduce your screening burden - If you have embarked on protein engineering projects before, then you know the hardships of seemingly endless screening efforts.
Random mutagenesis approaches have led to amazing discoveries. Yet, for all their strengths, the main caveat remains…. Huge mutant libraries requiring extensive, well-designed, and carefully executed selection methods. An almost herculean task!
Possu Huang, Ph.D.
Assistant Professor, Stanford University
Choose your codons - Ever wished you could make only desired changes in unique protein domains to more quickly and directly test your hypothesis?
Whether you want to change a specific codon, contiguous codons, or multiple codons in different protein regions, precise control over oligonucleotide synthesis allows you to test only desired mutations. Absolute control over oligonucleotide synthesis has the added benefit of eliminating stop codons that would otherwise result in fruitless pursuits during screening.
Meredith Jackrel, Ph.D.
Assistant Professor, Washington University
Avoid bias - One of the biggest challenges when creating libraries with degenerate codons (e.g., NNK or NNS; where N is A, C, G, or T, K is G or T, and S is C or G) is redundancy, which results in mutant libraries with skewed amino acid distribution.
You may blame the redundancy of the genetic code, where amino acid representation is unequal. Ultimately, while degenerate oligos diminish redundancy, resulting libraries are still biased, and without careful screening, unique variants may be missed.
Are you ready to take these unparalleled advantages offered by our Precision Mutant Libraries (PMLs)? Visit us at Explore PML Service!
