GenCircle dsDNA_Sleeping Beauty_CMV_CD19_GFP

A sequence encoding CD19CAR_T2A_GFP expression cassette with Sleeping beauty ITRs, transcriptionally promoted by an independent CMV promoter.

Description

A sequence encoding CD19CAR_T2A_GFP expression cassette with Sleeping beauty ITRs, transcriptionally promoted by an independent CMV promoter.

Storage Condition

Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Quality Specifications

Item	Assay	Specifications
Concentration	Spectrometry A260	Guaranteed quantity
Identity	Sanger Sequencing	Correct sequence
Identity	Restriction Analysis	DNA bands of expected sizes detected by agarose gel electrophoresis (AGE)
Purity	Spectrometry A260/280	1.8-2.0
Plasmid Topology	Agarose Gel Electrophoresis	≥ 90% supercoil
Bioburden	Innoculation Test	No bacteria/ fungi growth after 72 hours
Endotoxin	Gel Clot Test	≤0.01EU/μg
Residual Host RNA	Agarose Gel Electrophoresis	No bands detected
Residual Host DNA	AGE Gray Scale Analysis (GIS)	Non-detectable (GIS=0)