Figure 1. Schematic diagram of the chemical structure of Ni IDA Resin, Ni IDA Resin FF, Hi affinity Ni-Charged Resin and Hi affinity Ni-Charged Resin FF

High Affinity Ni-Charged Resin FF

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins (see Figure 1) [1]. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

Cat. No.	L00666
Size	5 ml Resin (Total 10 ml) 25 ml Resin (Total 50 ml) 100 ml Resin (Total 200 ml) Customized Size
Quantity
Price	$64.00


Ask us a question

Description

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins (see Figure 1) ^[1]. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

Overview

Product Information

Cat. No.	Product Name	Volume supplied	Resin content
L00666-5	High Affinity Ni-Charged Resin FF 5 ml	10 ml	50% slurry
L00666-25	High Affinity Ni-Charged Resin FF 25 ml	50 ml	50% slurry
L00666-100	High Affinity Ni-Charged Resin FF 100 ml	200 ml	50% slurry

Key Features

Characteristics of High Affinity Ni-Charged Resin FF

Matrix spherical	6% highly cross-linked agarose
Average particle size	90 µm (45-165 µm)
Dynamic Binding capacity¹	≥50 mg of histidine-tagged protein /mL settled resin
Storage solution	20% ethanol

¹ Dynamic binding capacity conditions:
Sample: 5 mg/mL (histidine)₆-tagged pure proteins (M_r 43 000) in binding buffer (capacity at 10% Breakthrough)
Column volume: 1 mL
Retention time: 3min
Flow rate: 0.333 mL/min
Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4
Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4
Note: Dynamic binding capacity is protein-dependent.

Reagents Compatible with High Affinity Ni-Charged Resin FF

Reducing agents	Denaturants	Detergents	Salts	Others
1 mM DTT	6 M Gua·HCl^†	2% Triton X-100	4 M MgCl₂	50% glycerol
20 mM β-ME	8 M Urea^†	2% Tween 20	5 mM CaCl₂	20% ethanol
5mM TCEP-HCl	——	50% glycerin	2 M NaCl	1 mM EDTA^‡
10 mM reduced glutathione	——	——	100mM Na₂SO₄	60 mM citrate^†

^†Tested for 1 week at 40°C.
^‡The strong chelator EDTA has been used successfully in some cases at 1 mM. Generally, chelating agents should be used with caution (and only in the sample, not in buffers). Any metal ion stripping may be counteracted by addition of a small excess of MgCl2 before centrifugation/filtration of the sample. Note that stripping effects may vary with applied sample volume.

Examples

Figure 1. Schematic diagram of the chemical structure of Ni IDA Resin, Ni IDA Resin FF, Hi affinity Ni-Charged Resin and Hi affinity Ni-Charged Resin FF

Properties

Storage

Store in 20% ethanol at 2°C to 8°C. Do not freeze.

High Affinity Ni-Charged Resin FF

SUPPORT

COMPANY

SUPPORT

COMPANY