CytoSinct™ Streptavidin Nanobeads

The CytoSinct Streptavidin Nanobeads are used for in vitro enrichment or depletion of cells labeled with one or a cocktail of biotinylated antibody or ligand from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension.

Price	$105.00
Cat. No.	L00898-0.5
Size	500 μl 1 ml Buy in bulk
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Overview Properties Bottom Notes

Overview

Product Description

The CytoSinct Streptavidin Nanobeads are used for in vitro enrichment or depletion of cells labeled with one or a cocktail of biotinylated antibody or ligand from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension.
To begin the isolation, biotinylated primary antibody or ligand are added to the cells for labeling, then CytoSinct Streptavidin Nanobeads are added. The ceils/beads mixed suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or other compatible columns with a magnet.
The cells labeled with Nanobeads are retained within the column and unlabeled cells are flushed out during the wash step. After removing the column from the magnetic field, the retained labeled cells can be eluted as the positively selected cell fraction.

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Properties

Format

Supplied in water.

Components

1 mL or 0.5 mL CytoSinct Streptavidin Nanobeads
Nanobeads conjugated to streptavidin protein.

Volume

Cat. No.	Name	Size	Capacity
L00898-0.5	CytoSinct™ Streptavidin Nanobeads	500 μL	for up to 5×10⁸ total cells
L00898-1	CytoSinct™ Streptavidin Nanobeads	1 mL	for up to 1×10⁹ total cells

Storage & Stability

Store at 2-8 °C. Do not freeze.

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T cells were enriched from human PBMCs using CytoSinct Streptavidin Nanobeads (Cat. L00898) and biotinylated anti-human CD3 antibody. CD3⁺ cells were stained with anti-CD3 (clone: OKT3) BV785 and gated on Live/hCD45⁺. »

For research use only. Not intended for human and animal therapeutic or diagnostic use.