Streptavidin MagBeads

Intended Use
GenScript Streptavidin MagBeads are ideal for fast and convenient purification of biotinylated proteins, peptides, antibodies and oligonucleotides, and protein interaction studies.

Principle
Add the sample containing biotinylated molecules to the Streptavidin MagBeads and allow the molecules bind to the MagBeads during a short incubation. Then separate the molecule‐bound beads with a magnetic separation rack. With indirect capture, mix the biotinylated molecule with the sample to capture the molecule‐target complex before adding the MagBeads.

Description of Material
GenScript Streptavidin MagBeads are super paramagnetic beads of approximately 45-100 μm in diameter, covalently coated with highly purified streptavidin. These magnetic beads are composed of magnetite core encapsulated by agarose. The magnetic beads have streptavidin as its surface protein which is 53kDa. in size having four binding site per molecule. These beads have pH stability of 2 to 14 (short term); 3 to 13 (long term). The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol.

Storage and Stability
This product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination.