StrepCaptureXP Resin FF

GenScript StrepCaptureXP Resin FF (Cat. No. L01091) is designed for the purification of Strep-tag®II and Twin-strep-tag® proteins from commonly used protein expression systems including bacteria, yeast and mammalian cells.

Price	$105.00
Cat. No.	L01091-1
Size	1 ml Resin (Total 2 ml) 5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) Buy in bulk
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Overview Properties Terms of Use Bottom Notes

Overview

Product description

The Strep-tag® II (WSHPQFEK) and Twin-Strep-tag® (WSHPQFEKGGGSGGGSGGSAWSHPQFEK) are widely adopted affinity tags for the purification of recombinant proteins in basic research, diagnostics, and biomanufacturing.
GenScript’s StrepCaptureXP Resin FF (Cat. No. L01091) is a next-generation affinity chromatography medium engineered for the efficient purification of Strep-tag® II and Twin-Strep-tag® fusion proteins. Functionalized with an innovatively engineered StrepCaptureXP ligand—a genetically optimized streptavidin variant—this resin enables high-affinity, high-specificity capture proteins from complex biological lysates, including those derived from mammalian, bacterial, and insect expression systems.
The resin's high dynamic binding capacity, combined with mild and reversible elution conditions, ensures maximal recovery of biologically active proteins. It is especially suitable for applications requiring high purity and gentle handling, such as in the purification of fragile multi-subunit complexes, or therapeutic candidates.

Key Features

● High specificity and low background binding.
● Compatible with neutral pH and mild elution buffers.
● Robust performance across a variety of expression hosts.
● Optimized for gravity flow, FPLC, and automated purification systems.

Selection Guide

	StrepCaptureXP Magnetic Beads (Cat. No.: L01075)	StrepCaptureXP Resin FF (Cat. No.: L01091)	StrepCaptureXP Resin Lite (Cat. No.: L01114)
Recommended Applications	High-throughput screening & small-scale purification	Scalable purification & process development	Routine research purification & small-scale protein preparation
If You Need	✓ High-throughput screening ✓ Automated parallel purification ✓ AI-driven protein screening and parallel evaluation of multiple protein candidates ✓ Fast turnaround and high first-pass success rate	✓ Scale-up purification ✓ Compatible with ÄKTA™ systems ✓ High stability for repeated purification cycles ✓ Cost-effective for long-term manufacturing and process development	✓ Flexible for small-scale protein preparation ✓ Easy-to-use format for manual purification ✓ Lower entry cost for routine research applications ✓ Challenging proteins including membrane proteins
Recommended Workflow	Automated magnetic purification platforms and parallel purification workflows	ÄKTA™ chromatography systems and scalable purification processes	Manual purification or small-scale chromatography workflows
Typical Scale	Small volume purification (Recommended: <1 mL beads equivalent)	Medium- to large-scale purification	Small-scale laboratory purification
Automation Compatibility	Compatible with automated magnetic purification platforms (e.g., AmMag™ Quatro 1300 )	Compatible with ÄKTA™ chromatography systems	Compatible with commonly used chromatography systems, including ÄKTA™ systems under recommended operating conditions (refer to Product Manual)
Binding Capacity	20–40 mg/mL beads	10–30 mg/mL resin	8–15 mg/mL resin
Reusability	≥30 purification cycles	≥100 purification cycles	≥35 purification cycles
Purity of Eluted Protein	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)

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Properties

Product Content	50% settled resin suspended in PBS (pH 7.2) with 0.1% ProClin300
Base Matrix	4% highly cross-linked agarose
Particle Size	45-165 μm
Pressure	0.3 MPa
Affinity Ligand	StrepCaptureXP
Binding Capacity	10-30 mg protein/mL settled resin. (Please note that the binding capacity is protein dependent)
Elution Method	Competitive elution with 50 mM Biotin
Reuse Capability	≥100 purification cycles when regenerated and stored as recommended.
Reagents Compatibility	Compatible with commonly used lysis reagents.
Storage	This product is stable for 1 year if stored at 2°C~8°C from the date of manufacture. Do not store at 0°C or below 0°C to avoid freezing the resin.

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Terms of Use

All product and company names mentioned, such as Strep-tag® or Twin-strep-tag® (registered trademarks of IBA Lifesciences GmbH), are the property of their respective owners and GenScript does not have sponsorship or endorsement from IBA. They are used for identification purposes only.

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.