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| M00951 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress Homo sapiens erb-b2 receptor tyrosine kinase 3 (ERBB3, HER3) on the surface. The surface expression of HER3 is validated by FACS analysis. |
| Culture Properties | Adherent |
| Stability | Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS |
| Freeze Medium-DATA | 90% FBS (Cat. No. 10099-141, Life Technologies), 10% (V/V) DMSO (Cat. No. D2650, Sigma) |
Figure 1. FACS analysis of human HER3 expression in CHO-K1/HER3 cells. The CHO-K1/HER3 cells (Red) and the negative control CHO-K1 cells (Green) were probed using HER3 antibody Patritumab (Cat. No. HY-P99275, MCE) and Goat anti-human IgG Antibody (Cat. No. A21445, Invitrogen). »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
