Protein Expression & Purification
For over a decade now, GenScript has been an industry-recognized leader in providing high quality recombinant protein expression and purification services, with over 5,300 high purity recombinant proteins delivered to its customers worldwide. GenScript offers a variety of protein expression and purification platforms and has driven the development of several improvements in protein expression and purification technologies, involving bacterial, yeast, insect and mammalian expression systems.
Our Recombinant Protein Expression & Purification Systems overview table below explains the major pros and cons of each system. Click your desired protein expression & purification system in the table to learn more about your chosen expression system and our related services.
Recombinant Protein Expression & Purification Systems
System |
Strain Used |
Pros |
Cons |
Intended Protein Applications |
Bacterial
protein expression and purification |
E.coli |
- Relatively inexpensive
- Simple genetics
- Easy to manipulate
- Easy scale up
- Fast expression
|
- Lack of efficient post-translational modifications
- Codon usage issues
- Inclusion bodies
- Low yield and activity for some eukaryotic proteins
- Difficult to express higher MW proteins
|
- Functional assays
- Antigen production
- Protein-protein interaction (Bacterial & Yeast expression systems recommended)
- Therapeutic protein (Yeast & Mammalian expression systems recommended)
|
Yeast
protein expression and purification |
Pichia pastoris, Saccharomyces cerevisiae |
- Diverse post-translational modifications
- Low cost of culture media
- Industry-scale fermentation
|
- Improper glycosylation
- Excessive glycosylation
|
Insect
protein expression and purification |
Sf-9, Sf-21,
S-2, Hi-5 |
- Good secretion
- Post translational modifications resemble mammalian system
- Suitable for toxic gene products
|
- Long production time
- Relative high media costs
|
Mammalian
protein expression and purification |
CHO,
HEK-293 |
- Comprehensive post-translational modifications
- Excellent method for the production of bioactive proteins
|
- Long production time
- High media costs
- Protein yields relatively lower
|
GenScript also offers a host of other services like chemical protein synthesis, protein refolding etc. GenScript's greatest asset is its customizable, full-spectrum service offering that can take your project all the way from gene sequence to pure, high quality protein. With world class, PhD-level technical expertise to monitor your projects, flexible production scale, and adoption of in-house proprietary technologies, GenScript works in partnership with you to ensure the best service at all times.
Advantages of our Protein Expression & Purification Services
- Comprehensive expression systems: Bacteria, Yeast, Baculovirus/Insect cell and Mammalian cell plus various vectors for each system.
- Fast turnaround: Our Upgraded BacPower™ Guaranteed Package delivers 3 mg of purified protein in as little as 4 weeks merely from your submitted target gene sequence, no protein - no charge.
- Bioprocess services: Significantly increase the expression level of your target protein to gram level.
- One-stop service platform: GenScript can take your project directly from gene synthesis to recombinant protein expression, stable cell line generation, antibody production and assay development.
Customer Testimonial for our Protein Expression & Purification Services
"GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service."
— Dr. Barry Bradford, Kansas State University, Department of Animal Sciences & Industry
Quotations and Ordering