Catalog Products » Protein Detection and Immunoassay » Reagents for Protein Analysis » Streptavidin-HRP

Streptavidin-HRP

Streptavidin is a protein that has similar binding properties to avidin. It is isolated from streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 da water soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10 - 15 ) for biotin. Streptavidin - HRP (M00091) is a HRP conjugated streptavidin and can be used for analysis of biotinylated molecules.
M00091
$95.00

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Properties
Formulation Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.
Reconstitution Reconstitute the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final concentration of 1 mg/ml.
Storage The reagent may be stored at 4°C for short periods of one month or less. For longer periods,aliquot and store at -20°C . Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.

Applications
Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points for this product:
Dot blot: 1:2,000-1:10,000
Western blot: 1:2,000-1:20,000
ELISA: 1:5,000~1:20,000
Others: User-optimized

Background
Target Background Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin.
Streptavidin-HRP (M00091) is coupled steptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.

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