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FITC-Protein L

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.
$105.00
M00920-500

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Overview
Species Peptostreptococcus magnus
Expression System E. coli
Tag His Tag
Conjugate FITC
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Applications
The optimal dilution ratio should be determined by the end user for specific applications.
Application Recommended Usage
Flow cytometry analysis 1:100-1:200
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Properties
Purity > 90% as analyzed by SDS-PAGE
Formulation Lyophilized from a solution in PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder in deionized water up to 0.5 mg/ml.
Theoretical Molecular Weight 42 kDa
Apparent Molecular Weight ~45 kDa, on SDS-PAGE under reducing conditions
Storage Upon receiving, this product remains stable for 12 months at -20°C or below. Upon reconstitution, the product can be stored for 2-3 weeks at 2-8°C or 3 months at -20°C. Avoid repeated freeze-thaw cycles.
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FITC-Protein L

VHH expression positive cell line was stained with negative control (black curve) or Protein L labeled with FITC at 5 μg/mL (red curve). »

FITC-Protein L

Lane 1: 1 μg of Protein L, reducing(R)
> 90% as analyzed by SDS-PAGE »

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Background
Synonyms RPL; Protein L
Target Background Protein L is a cell surface protein from Peptostreptococcus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.
References 1. Björck, L. "Protein L. A novel bacterial cell wall protein with affinity for Ig L chains." The Journal of Immunology 140.4 (1988): 1194-1197.
2. Kastern, William, U. Sjöbring, and L. Björck. "Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain." Journal of Biological Chemistry 267.18 (1992): 12820-12825.
3. Åkerström, B., and L. Björck. "Protein L: an immunoglobulin light chain-binding bacterial protein: characterization of binding and physicochemical properties." Journal of Biological Chemistry 264.33 (1989): 19740-19746.
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For research use only. Not intended for human and animal therapeutic or diagnostic use.