Catalog Products » Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
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WRN

Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates, and also 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. Plays a role in double-strand break repair after gamma-irradiation.
$220.00
RP-D00003-20

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Description

Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates, and also 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. Plays a role in double-strand break repair after gamma-irradiation.
Background
Synonyms Werner syndrome helicase, RECQ3, RECQL2, RECQL3, WRN RecQ like helicase, Werner syndrome RecQ like helicase
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Product Introduction
Species Homo sapiens
Construct Full length
Appearance Clear liquid
Accession# Q14191
Purity ≥ 85% by SDS-PAGE
Endotoxin Level ≤ 100 EU/mg
Source Insect cell Sf9
Theoretical Molecular Weight 162 kDa
Tag His tag at the C-terminus
Application Drug discovery for cancer therapeutics. In vitro affinity assays, activity assays etc.
Formulation Supplied as a solution of 50 mM Tris, 300 mM NaCl, 10% Glycerol, 2 mM TCEP, pH 8.0
Storage & Stability This product remains stable for up to 12 months at -80±5°C. Avoid repeated freeze-thaw cycles.
Note For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
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WRN

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