CFXpress™ PLUS E.coli Cell-free Protein Synthesis Kit

A ready-to-use, high-yield E. coli-based cell-free protein synthesis system with built-in T7 RNA polymerase for rapid expression from plasmid or linear DNA with T7 promoter.

Price	$120.00
Cat. No.	RP-D00006-1
Size	1 ml 5 ml Buy in bulk
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Product Introduction

Description

The GenScript CFXpress™ Plus Cell-free E. coli Protein Synthesis Kit is a ready-to-use platform optimized for rapid, high-yield in vitro synthesis of proteins with disulfide bonds.
The kit consists of three main components:
• Cell Extract
• Protein Synthesis Buffer
• Control Plasmid
• DB Oxidizer
• DB Reducer

By following the provided protocol and combining Cell Extract and Protein Synthesis Buffer with your target templates, protein expression can be achieved within a few hours. The system supports both clonal and non-clonal DNA templates. Custom formulation guidance is included for expressing proteins with multiple disulfide bonds, promoting correct folding and improved functional yield. Synthesized proteins are compatible with downstream characterization, purification, and functional assays. The system is compatible with high-throughput automation workflows and dialysis method.

Product Introduction

Kit components

Size	Component	Quantity	Storage	Catalog #
1 mL (20 reactions)	Cell Extract Plus (red cap)	1 (370 µl)	-80°C	RP-D00006-1
	Protein Synthesis Buffer (clear cap)	1 (600 µl)	-20°C or -80°C
	DB Reducer, 0.1 M (green cap)	1 (1 mL)	-20°C or -80°C
	DB Oxidizer, 0.1 M (yellow cap)	1 (1 mL)	-20°C or -80°C
	Control Plasmid (pIVEX-sfGFP), 0.5 µg/µL (blue cap)	1 (7.5 µg)	-20°C or -80°C
5 mL (100 reaction)	Cell Extract Plus (red cap)	5 x (370 µL)	-80°C	RP-D00006-5
	Protein Synthesis Buffer (clear cap)	5 x (600 µL)	-20°C or -80°C
	DB Reducer, 0.1 M (green cap)	1 (1 mL)	-20°C or -80°C
	DB Oxidizer, 0.1 M (yellow cap)	1 (1 mL)	-20°C or -80°C
	Control Plasmid (pIVEX-sfGFP), 0.5 µg/µL (blue cap)	2 x (7.5 µg)	-20°C or -80°C

Application

• Rapid expression and screening of disulfide-bonded proteins, including mini binders, VHHs, and scFvs fragements
• Fast AI-driven enzyme design and testings
• Cell-toxic protein expressions

Storage & Stability

- 80℃, avoid repeated freeze–thaw

Note

For research use only

Template Guidelines

Please refer to the user manual for detailed information.
We recommend our cloning vectors: pT7-N-His and pT7-C-His for constructs requiring His-tagged proteins. For additional information on available vectors, refer to our GenScript Vector Library.

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