|Synonyms||Human Epidermal Growth Factor|
|Description||Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through the EGF receptor (EGFR) also known as erbB1, is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents.
Recombinant Human EGF produced in E. coli is a 6,200 Da protein containing 53 amino acid residues.
|Biological Activity||The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg.|
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG
|Measured Molecular Weight||6.2 kDa+/-10% determined by reduced SDS-PAGE|
|Purity||Greater than 95% as determined by
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
|Dimers||Less than 1% as determined by silver-stained SDS-PAGE gel analysis|
|Formulation||Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.|
|Reconstitution||It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.|
|Quantitation||Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard.
|Endotoxin Level||Less than 0.2 EU/μg of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test.|
|Sequence Analysis||The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met- Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys.|
|UV||The maximal absorption wave is 275+/-3 nm.|
|QC||(a). The maximal absorption wave is 275+/-3 nm.
(b). Dimmers are Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
|Storage||Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.|
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