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Catalog Products » Recombinant Proteins » Growth Factors » Epidermal Growth Factor (EGF), Human

EGF, Human

EGF, Human

Biological Activity
EGF, Human (Cat. No. Z00333) stimulates cell proliferation of BALB/c 3T3 cells. The ED50 for this effect is typically 5–100pg/mL.

EGF, Human

GenScript product showed equal activity compared to competitor P

Synonyms Human Epidermal Growth Factor
Description Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through the EGF receptor (EGFR) also known as erbB1, is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents.
Recombinant Human EGF produced in E. coli is a 6,200 Da protein containing 53 amino acid residues.
Species Human
Source E. coli
Biological Activity The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg.
Sequence
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG
ERCQYRDLKW WELR

Measured Molecular Weight 6.2 kDa+/-10% determined by reduced SDS-PAGE
Purity Greater than 95% as determined by
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Dimers Less than 1% as determined by silver-stained SDS-PAGE gel analysis
Formulation Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.
Reconstitution It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Quantitation Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard.
Endotoxin Level Less than 0.2 EU/μg of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test.
Sequence Analysis The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met- Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys.
UV The maximal absorption wave is 275+/-3 nm.
QC (a). The maximal absorption wave is 275+/-3 nm.
(b). Dimmers are Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Storage Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

  • EGF, Human
  • EGF, Human

    Biological Activity
    EGF, Human (Cat. No. Z00333) stimulates cell proliferation of BALB/c 3T3 cells. The ED50 for this effect is typically 5–100pg/mL.

  • EGF, Human
  • EGF, Human

    GenScript product showed equal activity compared to competitor P


Katagiri S, Takahashi Y. Potential relationship between normalization of endometrial epidermal growth factor profile and restoration of fertility in repeat breeder cows. Anim. Reprod. Sci. Sep 2006; 95(1-2): 54-66.

Endo K, et al. Evaluation of the epidermal growth factor receptor gene mutation and copy number in non-small cell lung cancer with gefitinib therapy. Oncol. Rep. Sep 2006; 16(3): 533-541.

Li W, .et al PET imaging of EGF receptors using [18F] FBEM-EGF in a Head and Neck Squamous Cell Carcinoma model. Eur J Nucl Med Mol Imaging. 2012 Feb;39(2):300-8.

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Cat. No. Z00333
Size
Price
10 ug $25.00
50 ug $75.00
1 mg $140.00