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Catalog Products » Recombinant Proteins » Growth Factors » Stem Cell Factor (SCF), Human

SCF, Human

Synonyms rHuSCF; SCF
Description Human Stem Cell Factor (SCF), produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18,409 Da. Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. Stem Cell Factor (SCF) stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Species Human
Source E. coli
Biological Activity The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5×105 IU/mg.

Measured Molecular Weight 18,409 Da
Purity Greater than 95.0% as determined by the following methods:
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Formulation The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH4.5, 200mM NaCl buffer.
Reconstitution It is recommended that the lyophilized recombinant human stem cell factor (SCF) be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin Level Less than 0.1 ng/µg (IEU/µg) of rHuSCF
Sequence Analysis The sequence of the first five N-terminal amino acids has been found to be Met-Glu-Gly-Ile-Cys.
Storage Lyophilized recombinant human stem cell factor (SCF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution Recombinant Human Stem Cell Factor (SCF) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.
Note ;


Cat. No.
Product Name
Price
Z02692-50
SCF, Human (P. pastoris-expressed) - 50 ug
$159.00
Z02692-10
SCF, Human (P. pastoris-expressed) - 10 ug
$85.00
Z02692-1
SCF, Human (P. pastoris-expressed) - 1 mg
$1200.00
 
Lili W, et al. Expression, Renaturation and Simultaneous Purification of Recombinant Human Stem Cell Factor in Escherichia coli. Biotechnol. Lett. Jul 2006; 28(13): 993-997.

Baccarani U, et al. Implication of stem cell factor in human liver regeneration after transplantation and resection. Growth Factors. Jun 2006; 24(2): 107-110.

Da Silva CA, et al. Effect of glucocorticoids on stem cell factor expression in human asthmatic bronchi. Clin. Exp. Allergy. Mar 2006; 36(3): 317-324.

Philip G,. et al High-resolution kinetics of cytokine signaling in human CD34/CD117-positive cells in unfractionated bone marrow. Blood.2011 Apr 14;117(15):e131-41.

Herraiz C, .et al Signaling from the Human Melanocortin 1 Receptor to ERK1 and ERK2 Mitogen-Activated Protein Kinases Involves Transactivation of cKIT. Mol Endocrinol.2011 Jan;25(1):138-56.

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Cat. No. Z00400
Size
Price
1 mg $2100.00