Catalog Products » Recombinant Proteins » Enzymes and Inhibitors » Enterokinase, Light Chain, Porcine

Enterokinase, Light Chain, Porcine

*This product has been discontinued!*
GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.
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Description

GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.

Synonyms

Enterokinase; EK

Note

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Overview
Source P. pastoris
Species Porcine

Properties
Biological Activity One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.
Concentration 50 ul, 2 U/ul
Formulation GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.
Measured Molecular Weight Theoretical MW: 21,880 Da
The apparent MW on SDS-PAGE: about 40,000 Da
Storage & Stability Store at -20°C after delivery.

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