|Description||GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.|
|Biological Activity||One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.|
|Measured Molecular Weight||Theoretical MW: 21,880 Da
The apparent MW on SDS-PAGE: about 40,000 Da
|Formulation||GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.|
|Concentration||50 ul, 2 U/ul|
|Storage||Store at -20°C after delivery.|
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