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Catalog Products » Recombinant Proteins » Other Catalog Proteins » Protein G

Protein G

Synonyms Protein G; G Protein
Description Protein G, a cell surface protein of group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A from Staphylococcus aureus. This product contains three IgG binding domains, but its albumin-binding region has been removed. Additionally, His-tag and cysteine have been added to N- and C-termini, respectively, to facilitate purification and conjugation. This does not affect the protein's ability to bind to IgG.
Source E. coli
Biological Activity The theoretical binding capacity is 3 mol IgG/mol protein G. Protein G is assayed using ELISA. The optimal concentration for coating plate is 5 ug/ml.

Measured Molecular Weight 29,000-35,000 Da was determined by SDS-PAGE while relative molecular mass calculated from amino acid sequence is ≈24,000 Da.
Purity This product is chromatographically purified and shows predominantly a single band by SDS-PAGE.
Focusing: pI of 4.69
Concentration 5 mg/ml before lyophilization
Storage This product is lyophilized in 1X PBS. It will remain stable at 4°C for several weeks. For longer periods, store it at -20°C.
Note ;

GenScript Protein G is suitable for use as antigen, as a control or standard in assays, for conjugation, and most other immunological methods.

  • Protein G
  • Protein G


Cat. No.
Product Name
Price
M00090
HRP-Protein G - 1 mg
$75.00
L00209
Protein G Resin - 5 ml
$129.00
 

Bodelón G., et al. Nickel Nanoparticle-Doped Paper as a Bioactive Scaffold for Targeted and Robust Immobilization of Functional Proteins. ACS Nano. 2014June;8(6):6221-31

Nitsche-Schmitz DP, et al. Group G Streptococcal IgG Binding Molecules FOG and Protein G Have Different Impacts on Opsonization by C1q. J. Biol. Chem. Jun 2007; 282(24): 17530-17536.

Kang M, et al. G-protein coupled receptor signaling in myocardium: not for the faint of heart. Physiology (Bethesda). Jun 2007; 22(3): 174-184.

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Cat. No. Z02007
Size
Price
5 mg $95.00