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Catalog Products » Recombinant Proteins » Growth Factors » Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Human (P. pastoris-expressed)

GM-CSF, Human (P. pastoris-expressed)

Synonyms rHu GM-CSF, Sargramostim, Recombinant Human GM-CSF, Sargramostim;
Description Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
GenScript Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Sargramostim), is a single, glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of about 26,000-32,000 Da, as shown in SDS-PAGE. It differs from natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein.
Species Human
Source P. pastoris
Sequence
The sequence of the
first five N-terminal amino
acids was determined and
was found to be
Ala-Pro-Ala-Arg-Ser.

Specificity The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg.
Measured Molecular Weight 26,000-32,000 Da
Purity The purity level of GenScript Recombinant Human GM-CSF is greater than 95.0%, as determined by the following methods:
(a) RP-HPLC anaylsis
(b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis
Formulation The protein was lyophilized after extensive dialysis against 10mM Tris-HCl, pH8.5, 4% Mannitol, 1% Sucrose buffer.
Reconstitution It is recommended to reconstitute the lyophilized Sargramostim in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin Level Less than 0.1 ng/µg (1 EU/µg) of sargramostim
Storage Although lyophilized GenScript Sargramostim can remain stable at room temperature for three weeks, it is best stored desiccated below -18°C. Upon reconstitution Sargramostim should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Note ;




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Cat. No. Z02190
Size
Price
10 ug $95.00
50 ug $325.00
1 mg $2250.00