|Synonyms||Interleukin (IL)-16, human;|
|Description||IL-16 is a CD8+ T cell-derived cytokine that induces chemotaxis of CD4+ T cells and CD4+ monocytes and eosinophils. Analysis by gel filtration suggests that, under physiological conditions, human IL-16 exists predominantly as a noncovalently linked multimer, but that some IL-16 may exist as a monomer. However, only the multimeric form appears to possess chemotactic activity, suggesting that receptor cross-linking may be required for activity. IL-16 also induces expression of IL-2 receptor (IL-2R) and MHC class II molecules on CD4+ T cells. Human and murine IL-16 show significant cross-species reactivity.
GenScript Interleukin (IL)-16, human produced in E. coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 13,400 Da.
The sequence of the
first five N-terminal amino
acids was determined and
was found to be
|Specificity||rHuIL-16 is fully biologically active when compared to standard. The ED50 as determined by its ability to chemoattract human CD4+ T lymphocytes using a concentration range of 10.0-100.0 ng/ml, corresponding to a specific activity greater than 1.0×104 IU/mg.|
|Measured Molecular Weight||13,400 Da|
|Purity||Greater than 97.0% as determined by
(a) RP-HPLC analysis
(b) Anion-exchange FPLC
(c) Reducing and non-reducing SDS-PAGE Silver Stained gel analysis
|Formulation||rHuIL-16 was lyophilized from 1 mg/ml solution after extensive dialysis against 20 mM acetic buffer, pH 5.0, 150 mM NaCl and 0.5 mM DTT.|
|Reconstitution||It is recommended to reconstitute the lyophilized rHuIL-16 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.|
|Endotoxin Level||Less than 0.1 ng/µg (1 IEU/µg) determined by LAL test|
|Storage||Lyophilized rHuIL-16 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-16 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.|
For more documents, please visit "Technical Support".