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Catalog Products » Recombinant Proteins » Growth Factors » Platelet-Derived Growth Factor-BB (PDGF-BB), Human (P. pastoris-expressed)

PDGF-BB, Human (P. pastoris-expressed)

Synonyms Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF).
Description Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle, connective tissue, bone and cartilage cells, and some blood cells. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer.Recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) produced in Pichia pastoris is a glycosylated polypeptide. rhPDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids) and is obtained by proprietary chromatographic techniques at GenScript.
Species Human
Source P. pastoris
Biological Activity ED50 <3 ng/ml, measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, corresponding to a specific activity of >3.3 x 105 units/mg.
Sequence
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW
PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK
KPIFKKATVT LEDHLACKCE TVAAARPVT

Measured Molecular Weight 24.3kDa, observed by non-reducing SDS-PAGE.
Purity > 97% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against 10mM acetic acid.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level <1.0 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhPDGF-BB should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

  • PDGF-BB, Human (P. Pastoris-expressed)
  • PDGF-BB, Human (P. Pastoris-expressed)

    ED₅₀<3 ng/ml, measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, corresponding to a specific activity of >3.3 x 10⁵ units/mg.




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Cat. No. Z02529
Size
Price
10 μg $95.00
50 μg $265.00
1 mg $2100.00