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Catalog Products » Recombinant Proteins » Cytokines » Leukemia Inhibitory Factor (LIF), Human

LIF, Human

LIF, Human

Biological Activity
LIF, Human (Cat. No. Z02681) stimulates cell proliferation of R&D TF-1 cells. The ED50 for this effect is less than 0.2ng/mL.

LIF, Human

2 μg of LIF, Human (Cat. No. Z02681) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining

Synonyms D factor, MLPLI
Description Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.
Recombinant human Leukemia Inhibitory Factor (rhLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rhLIF has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P15018
Species Human
Source E. coli
Biological Activity ED50 < 0.2 ng/mL, measured by a cell differentiation assay using TF-I cells, corresponding to a specific activity of > 5 × 106 units/mg.
Sequence
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL
FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL
VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD
ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK
LGCQLLGKYK QIIAVLAQAF

Measured Molecular Weight 19.7 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Leukemia Inhibitory Factor (rhLIF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhLIF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only;

  • LIF, Human
  • LIF, Human

    Biological Activity
    LIF, Human (Cat. No. Z02681) stimulates cell proliferation of R&D TF-1 cells. The ED50 for this effect is less than 0.2ng/mL.

  • LIF, Human
  • LIF, Human

    2 μg of LIF, Human (Cat. No. Z02681) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining


Huang B, .et al A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under chemically defined conditions of dual kinase inhibition. PLoS One. 2011 6(9):e24501.

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Cat. No. Z02681
Size
Price
50 ug $225.00
10 ug $85.00
1 mg $1500.00