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Catalog Products » Recombinant Proteins » Cytokines » Tumor Necrosis Factor-alpha (TNF-α), Human (P. pastoris-expressed)

TNF-α, Human (P. pastoris-expressed)

Synonyms Tumor Necrosis Factor-alpha (TNF-alpha), Cachectin.
Description Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) produced in Pichia pastoris is a glycosylated polypeptide chain of 157 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 17.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species Human
Source P. pastoris
Specific Activity ED50<0.08ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >1.25 x 107units/mg.
Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG
VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI
SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL
GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Measured Molecular Weight 17.4kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level <1.0 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Note ;

  • TNF-α, Human (P. Pastoris-expressed)
  • TNF-α, Human (P. Pastoris-expressed)

    ED₅₀<0.08ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >1.25 x 10⁷units/mg.


Thuy TT,. et al Glycosylation Profiling of Therapeutic Antibodies in Serum Samples Using a Microfluidic CD Platform and MALDI-MS. J Am Soc Mass Spectrom. 2013 Jul;24(7):1053-63.

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Cat. No. Z02682
Size
Price
50 ug $95.00
10 ug $49.00
1 mg $625.00
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