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Catalog Products » Recombinant Proteins » Cytokine Bundles » Stem Cell Factor (SCF), Human (P. pastoris-expressed)

SCF, Human (P. pastoris-expressed)

Synonyms C-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor
Description Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.Recombinant human Stem Cell Factor (rhSCF) produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ~20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species Human
Source P. pastoris
Biological Activity The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5×105 IU/mg.
Sequence
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL
PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL
VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF
NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM
LPPVA

Measured Molecular Weight ~20 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against 10 mM acetic acid.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level <1.0 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Stem Cell Factor (rhSCF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhSCF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

  • SCF, Human (P. Pastoris-expressed)
  • SCF, Human (P. Pastoris-expressed)

    ED₅₀ < 2 ng/ml, as determined by the dose-dependent stimulation of human TF-1 cells, corresponding to a specific activity of 5×10⁵ IU/mg.




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Cat. No. Z02692
Size
Price
10 ug $85.00
50 ug $159.00
1 mg $1200.00