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Catalog Products » Recombinant Proteins » Growth Factors » Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Human (P. pastoris-expressed)

GM-CSF, Human (P. pastoris-expressed)

Synonyms Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897
Description Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds[2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities[4]. Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells[5].

Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species Human
Source P. pastoris
Biological Activity ED50 < 0.1ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 1.0x 107 units/mg.
Sequence
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET
VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM
MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF
DCWEPVQE

Measured Molecular Weight 26-32 kDa, observed by SDS-PAGE.
Purity > 95 % as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against 10 mM PB, pH7.0.
Reconstitution Reconstituted in ddH2O at 100μg/ml.
Endotoxin Level <1.0 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

  • GM-CSF, Human (P. Pastoris-expressed)
  • GM-CSF, Human (P. Pastoris-expressed)

    ED₅₀< 0.2ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 5x 10⁶ units/mg.


1. Hamilton, J. A. et al. (2005) Growth Factors. 22(4): 225–31.

3. Hansen, G.et al. (2008) Cell. 134:496-507.

5. Hercus, T. R. et al. (2012) Growth Factors. 30(2):63-75.

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Cat. No. Z02694
Size
Price
10 ug $85.00
50 ug $275.00
1 mg $2100.00