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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-10 (IL-10), Human

IL-10, Human

Synonyms Interleukin-10 (IL-10), Human;
Description Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of ª-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 . IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells . IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer . The IL-10 dimer binds to two IL-10 Rª/IL-10R1 chains, resulting in recruitment of two IL-10 Rß/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 . IL-10Rß does not bind IL-10 by itself but is required for signal transduction . IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation . It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
Species Human
Source E. coli
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Sequence
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK
DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH
VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM
SEFDIFINYI EAYMTMKIRN

Measured Molecular Weight Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuIL-10 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;


L Yang,.et al Recombinant Protein Yield In Rice Seed Is Enhanced By Specific Suppression Of Endogenous Seed Proteins At The Same Deposit Site. Plant Biotechnol J. 2012 Dec;10(9):1035-45.

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Cat. No. Z02707
Size
Price
10 ug $159.00
1 mg $3600.00
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