• English
  • Sign In
  • Contact Us
×
Catalog Products » Recombinant Proteins » Growth Factors » Vascular Endothelial Growth Factor 165 (VEGF165), Mouse

VEGF165, Mouse

Synonyms Vascular Endothelial Growth Factor 165 (VEGF165), Mouse;
Description Vascular Endothelial Growth Factor (VEGF) was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer ofKDR/Flt in VEGF signal transduction remain to be elucidated.In vivo, VEGF has been found to be a potent angiogenesis inducer.
Species Mouse
Source E. coli
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
Sequence
MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP
DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH
IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK
NTDSRCKARQ LELNERTCRC DKPRR

Measured Molecular Weight Approximately 38.8 kDa, a disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rMuVEGF165 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note


Cat. No.
Product Name
Price
Z02779-10
VEGF120, Mouse - 10 ug
$159.00
Z03140-10
VEGF121, Human - 10 ug
$85.00
Z02917-1
VEGF164, Rat (P. pastoris-expressed) - 1 mg
$1500.00
Z02690-20
VEGF164, Mouse (P. pastoris-expressed) - 20 ug
$95.00
Z02689-10
VEGF165, Human - 10 ug
$85.00
Z03073-10
VEGF165, Human(HEK 293-expressed) - 10 ug
$85.00
Z03006-10
VEGF165, Rat (CHO-expressed) - 10 ug
$85.00
Z03345-10
VEGF-A164, Mouse - 10 ug
$85.00
Z03286-10
VEGF-C, Human - 10 ug
$60.00
Z03125-10
VEGF-D, Human - 10 ug
$95.00
 



Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. Z02780
Size
Price
10 ug $159.00