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Catalog Products » Recombinant Proteins » Enzymes and Inhibitors » PreScission Protease (PSP)

PreScission Protease

Synonyms PreScission Protease(PSP);
Description PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease . The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues . Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.
Source E. coli
Biological Activity Unit Definition:
One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 90% completion at 5°C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Cleavage Buffer:
50 mM Tris-HCl, pH 7.0 (at 25 °C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 °C prior to use.

Reaction Buffer Cleavage Buffer:
50mM Tris-HCl, pH7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5°C prior to use.
Physical Appearance Sterile colorless liquid.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE
Storage Should be stored in small aliquots at -20°C to -80°C for long term.
Note This material is offered for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.


Cat. No.
Product Name
Price
Z03092-100
3C protease, His, Human - 100 IU
$39.00
 



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Cat. No. Z02799
Size
Price
5000 IU Quote
100 IU $75.00
250 IU $159.00