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Catalog Products » Recombinant Proteins » Other Catalog Proteins » SPARC, Human

SPARC, Human

Synonyms SPARC, Human;
Description SPARC, an acronym for ’secreted protein, acidic and rich in cysteine', is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid , 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.
Species Human
Source E. coli
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 μg/mL, corresponding to a specific activity of > 333 IU/mg.
Sequence
MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE
VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE
NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE
KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC
KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT
EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF
PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF
FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI

Measured Molecular Weight Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 × His tag).
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuSPARC, His as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;




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Cat. No. Z02804
Size
Price
50 ug $159.00
1 mg $1250.00