Catalog Products » Recombinant Proteins » Cytokines » Bone Morphogenetic Protein-2 (BMP-2), Human

BMP-2, Human

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Biological Activity
BMP-2, Human (Cat. No. Z02913) induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is typically 0.07-0.20 ug/mL.

Biological Activity
BMP-2, Human (Cat. No. Z02913) induce alkaline phosphatase production by C2C12 cells. The ED₅₀ for this effect is typically 0.10-0.80 ug/mL.

2 μg of BMP-2, Human (Cat. No. Z02913) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

GenScript product showed better activity compared to competitor P

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Overview

Synonyms	BMP-2, BMP2A, Bone morphogenetic protein 2, BMP-2A, BMP2
Description	Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met¹-Arg³⁹⁶, with a signal peptide from Met¹ to Gly²³), propeptide (Leu²⁴-Arg²⁸²) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys³⁶⁰. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction. Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 115 amino acids. A fully biologically active molecule, rhBMP-2 has a molecular mass of 26 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species	Human
Source	E. coli
Biological Activity	Assay #1: Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells, The ED₅₀ for this effect is typically 0.07-0.2 µg/mL. Assay #2: Measured by its ability to induce alkaline phosphatase production by C2C12 cells, The ED₅₀ for this effect is typically 0.2-1 µg/mL.
Sequence	MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Properties

Measured Molecular Weight	26 kDa, observed by non-reducing SDS-PAGE
Purity	> 95% as analyzed by non-reducing SDS-PAGE and HPLC analyses
Formulation	Lyophilized after extensive dialysis against 50 mM acetic acid.
Reconstitution	Reconstituted in 20 mM AcOH or 5 mM HCl. The solubility should be at 100 μg/ml.
Endotoxin Level	< 1 EU/μg, determined by LAL method.
Storage	Lyophilized recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhBMP-2 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note

Examples

Biological Activity
BMP-2, Human (Cat. No. Z02913) induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is typically 0.07-0.20 ug/mL.

Biological Activity
BMP-2, Human (Cat. No. Z02913) induce alkaline phosphatase production by C2C12 cells. The ED₅₀ for this effect is typically 0.10-0.80 ug/mL.

2 μg of BMP-2, Human (Cat. No. Z02913) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

GenScript product showed better activity compared to competitor P

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Expand all

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Cushnie EK., et al. Simple Signaling Molecules for Inductive Bone Regenerative Engineering. PLoS One. 2014July;9(7):e101627

E Alegre-Aguarón., et al. Growth Factor Priming Differentially Modulates Components of the Extracellular Matrix Proteome in Chondrocytes and Synovium-Derived Stem Cells. PLoS One. 2014Feb.;9(2):e88053

A Clark., et al. Mechanical Properties and Dual Drug Delivery Application of PLGA Scaffolds Fabricated with a Poly (β-amino ester) Porogen. Acta Biomater. 2014Jan.

Chanchareonsook N., et al Segmental mandibular bone reconstruction with a carbonate-substituted hydroxyapatite-coated modular endoprosthetic poly(ɛ-caprolactone) scaffold in Macaca fascicularis. J Biomed Mater Res B Appl Biomater. 2013 Nov 21.

John C., et al Design, fabrication and in vitro evaluation of a novel polymer‐hydrogel hybrid scaffold for bone tissue engineering. J Tissue Eng Regen Med. 2012 Jun 11.

Jeremy Bonor., et al Initiation of BMP2 signaling in domains on the plasma membrane.J Cell Physiol. 2012 Jul;227(7):2880-8.

Moseychuk O., et al Inhibition of CK2 binding to BMPRIa induces C2C12 differentiation into osteoblasts and adipocytes.J Cell Commun Signal. 2013 Dec;7(4):265-78.

Sven Saldanha., et al Caveolae regulate smad signaling as verified by novel imaging and system biology approaches. J Cell Physiol. 2013 May;228(5):1060-9.

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