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Catalog Products » Recombinant Proteins » Growth Factors » Vascular Endothelial Growth Factor 164 (VEGF164), Rat (P. pastoris-expressed)

VEGF164, Rat (P. pastoris-expressed)

Synonyms Vascular Endothelial Growth Factor 164 (VEGF-164), VEGF, Vascular Permeability Factor (VPF)
Description Vascular Endothelial Growth Factor A164 (VEGF-A164), a member of the cysteine knot growth factor[1], is one of major isoforms of VEGF-As. VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties[2]. During maturation, rat VEGF-A is alternatively spliced to generate rVEGF-A120, rVEGF-A164 and rVEGF-A188[3] which correspond to hVEGF-A121, hVEGF-A165 and hVEGF-A189 in human, respectively (the numbers designate the amino acid residues). The active form of rVEGF-A164 is either a homodimeric or heterodimeric polypeptides which bind to the transmembrane tyrosine kinases receptors FLT1, FLK1 or KDR[4] or to the non-tyrosine kinase neuropilin receptors NRP1/2[5].

Recombinant rat Vascular Endothelial Growth Factor A164 (rrVEGF-A164) produced in P. pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rrVEGF-A164 has a molecular mass of 38 kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P16612-2
Species Rat
Source P. pastoris
Biological Activity ED50<4 ng/ml, measured by cell proliferation assay of HUVEC.
Sequence
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP
DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI
MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPENHCEPC
SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC
DKPRR

Measured Molecular Weight 38 kDa, observed by non-reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level <1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant rat Vascular Endothelial Growth Factor A164(rrVEGF-A164) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrVEGF-A164 should be stable up to 1 week at 4°C or up to 2 months at -20°C.

  • VEGF164, Rat (P. Pastoris-expressed)
  • VEGF164, Rat (P. Pastoris-expressed)

    ED₅₀<4 ng/ml, measured by cell proliferation assay of HUVEC.


Cat. No.
Product Name
Price
Z02779-10
VEGF120, Mouse - 10 ug
$159.00
Z03140-10
VEGF121, Human - 10 ug
$85.00
Z02690-20
VEGF164, Mouse (P. pastoris-expressed) - 20 ug
$95.00
Z02689-10
VEGF165, Human - 10 ug
$85.00
Z03073-10
VEGF165, Human(HEK 293-expressed) - 10 ug
$85.00
Z02780-10
VEGF165, Mouse - 10 ug
$159.00
Z03006-10
VEGF165, Rat (CHO-expressed) - 10 ug
$85.00
Z03345-10
VEGF-A164, Mouse - 10 ug
$85.00
Z03286-10
VEGF-C, Human - 10 ug
$60.00
Z03125-10
VEGF-D, Human - 10 ug
$95.00
 
1. Tammela, T.et al. (2005) Trends Cell Biol. 15: 434-441.

4. Koch, S. et al. (2011) Biochem J. 437: 169-183.

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Cat. No. Z02917
Size
Price
2 ug $39.00
10 ug $85.00
1 mg $1500.00