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Catalog Products » Recombinant Proteins » Cytokines » Tumor Necrosis Factor-alpha (TNF-α), Mouse (P. pastoris-expressed)

TNF-α, Mouse (P. pastoris-expressed)

Synonyms TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2
Description Sharing 79% sequence identity with human Tumor Necrosis Factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4].

Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Species Mouse
Source P. pastoris
Biological Activity ED50<0.01ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 108units/mg.
Sequence
SQNSSDKPVA HVVANHQVEE QLEWLSQRAN ALLANGMDLK
DNQLVVPADG LYLVYSQVLF KGQGCPDYVL LTHTVSRFAI
SYQEKVNLLS AVKSPCPKDT PEGAELKPWY EPIYLGGVFQ
LEKGDQLSAE VNLPKYLDFA ESGQVYFGVI AL

Measured Molecular Weight 17kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level <1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmTNF-α should be stable up to 1 week at 4°C or up to 2 months at -20°C.


1. Friedmann, E. et al. (2006) Nat Cell Biol. 8: 843-48.

4. Hehlgans, T. and K. Pfeffer (2005)Immunology 115:1-20.

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Cat. No. Z02918
Size
Price
20 ug $85.00
100 ug $259.00
1 mg $1200.00