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Catalog Products » Recombinant Proteins » Cytokines » Beta-Defensin 1 (BD-1), Rat

BD-1, Rat

Synonyms Beta-defensin 1, BD-1, rBD-1, Defensin beta 1, Defb1.
Description Defensin are 3-4 kDa antimicrobial peptides of which three distinct families have been identified; α-defensin, β-defensins, and insect defensins. Rat β-Defensin 1, as a member of the β-defensin family, is a salt-sensitive antimicrobial peptide present in epithelia of the lung and urogenital tract. The predicted amino acid sequence shows the hallmark features of other known epithelial β-defensins, including the ordered array of six cysteine residues.
Species Rat
Source E. coli
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.

Measured Molecular Weight Approximately 4.1 kDa, a single non-glycosylated polypeptide chain containing 37 amino acids.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, 500 mM NaCl, pH 7.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rRtBD-1 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Cat. No. Z02946
5 ug $65.00
20 ug $159.00
1 mg $2100.00