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Catalog Products » Recombinant Proteins » Cytokines » Beta-Defensin 4 (BD-4), Rat

BD-4, Rat

Synonyms Beta-defensin 4, BD-4, BD-2, Defensin beta 4, RBD-2, RBD-4, Defb4, Defb2, Defb3.
Description Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four rat β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.
Species Rat
Source E. coli
Biological Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.
Sequence
QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK
K

Measured Molecular Weight Approximately 4.4 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 7.4, 500 mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rRtBD-4 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.




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Cat. No. Z02948
Size
Price
5 ug $65.00
20 ug $159.00
1 mg $2100.00