NANOG-TAT, Human

*This product has been discontinued!*

Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E. coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Cat. No.	Z03001
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

Expressed with additional C-terminal sequence (GGYGRKKRRQRRR).

NANOG-TAT [Met1-Val305 (Lys82Asn)]
Accession # Q9H9S0

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Expression System

E. coli

Apparent Molecular Weight

~36.2 kDa, on SDS-PAGE under reducing conditions.

Formulation

Supplied as a sterile filtered solution in 10 mM PB, 300 mM NaCl, pH 7.4.

Concentration

Please refer to the COA for the specific lot.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at -20°C or below and 1-2 weeks at 4°C. For long term storage, aliquot and store at -70°C or below. Avoid repeated freeze-thaw cycles.

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Background

Target Background

NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.

Synonyms

Homeobox protein NANOG; Homeobox transcription factor Nanog

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.