• English
  • Sign In
  • Contact Us
×
Catalog Products » Recombinant Proteins » Other Catalog Proteins » NANOG-TAT, Human

NANOG-TAT, Human

Synonyms None
Description NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.

Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E. coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Species Human
Source E. coli
Sequence
MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM
SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA
ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL
SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK
NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT
WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF
YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT
TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR

Measured Molecular Weight 36.2 kDa, analyzed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH7.4.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4°C from date of receipt. For long term storage, aliquot and store at lower than -70°C. Avoid repeated freezing and thawing cycles.
Note For research use only


Gideon Goldstein, et al.Exploratory clinical studies of a synthetic HIV-1 Tat epitope vaccine in asymptomatic treatment-naïve and antiretroviral-controlled HIV-1 infected subjects plus healthy uninfected subjects.Hum Vaccin Immunother. 2012Apr;8(4):479-85

You can visit "Citations Database" for more citations.






For more documents, please visit "Technical Support".

Cat. No. Z03001
Size
Price
100 ug $296.00