|Description||NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.
Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E. coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM
SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA
ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL
SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK
NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT
WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF
YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT
TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR
|Measured Molecular Weight||36.2 kDa, analyzed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH7.4.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4°C from date of receipt. For long term storage, aliquot and store at lower than -70°C. Avoid repeated freezing and thawing cycles.|
|Note||For research use only|
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