Enterokinase, His, Bovine

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.

Price	$60.00
Cat. No.	Z03004-100
Size	100 IU 500 IU 5000 IU Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Source	P. pastoris
Species	Bovine
Purity	> 95% as analyzed by reducing SDS-PAGE.
Biological Activity	5 IU/μl.
Unit Definition	One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.
Endotoxin Level	< 1.0 EU/μg, determined by gel clot method.
Molecular Weight	~40 kDa, observed by reducing SDS-PAGE.
Formulation	Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl₂, 50% glycerol, pH 7.4.
Components	100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol) 100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0) 3.6 ml EK Dilution/Storage Buffer (20mM TrisHCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol) 1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl₂)
Storage & Stability	Recombinant Bovine Enterokinase (rbEK) remains stable for 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity. Please avoid freeze-thaw cycles.

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Lane 1: 2 μg of Enterokinase, His, Bovine, non-reducing (NR)
Lane 2: 2 μg of Enterokinase, His, Bovine, reducing (R)
> 95% as analyzed by SDS-PAGE »

Background

Synonyms

Enteropeptidase, ENTK, PRSS7

Target Background

Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni²⁺ affinity chromatography and was designed for removing from digestion system.
Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Citations

1. CaiChao, et al. Three Genes Define a Bacterial-Like Arsenic Tolerance Mechanism in the Arsenic Hyperaccumulating Fern Pteris vittata. Curr Biol. (2019-05)