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Catalog Products » Recombinant Proteins » Enzymes and Inhibitors » Enterokinase (EK), His, Bovine

Enterokinase, His, Bovine

Synonyms Enteropeptidase, ENTK, PRSS7
Description Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Components:
  • 100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl,
    2mM CaCl 2, 50% glycerol)
  • 100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
  • 3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol)
  • 1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl₂)
  • Species Bovine
    Source P. pastoris
    Biological Activity 5 IU/μl.
    Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.
    Key Features Components:
    100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
    100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
    3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
    1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2)
    Storage: Store kit components at –20°C

    Measured Molecular Weight Theoretical MW: 22.7 kDa.
    Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
    Purity > 95% by SDS-PAGE analyses.
    Formulation Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
    Endotoxin Level < 1.0 EU/μg, determined by LAL method.
    Storage Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity.Please avoid freeze-thaw cycles.
    Note For research use only


    Cat. No.
    Product Name
    Price
    Z03376-1
    Enterokinase (EK), His, Lyophilized, Bovine - 1 mg
    $675.00
    Z01003
    Enterokinase, Light Chain, Porcine - 100 IU
    $65.00
    Z02199-100
    Enterokinase, Porcine - 100 IU
    $65.00
     

    NY Liu,.et alBinding properties of pheromone-binding protein 1 from the common cutworm Spodoptera litura.Comp Biochem Physiol B Biochem Mol Biol. 2012 Apr;161(4):295-302

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    Cat. No. Z03004
    Size
    Price
    100 IU $49.00
    500 IU $125.00
    5000 IU $775.00