|Synonyms||EDF, BCDFII, TRF|
|Description||Interleukin-5 (IL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.
Recombinant Human Interleukin-5 (rhIL-5) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 116 amino acids each. A fully biologically active molecule, rhIL-5 has a molecular mass of 26.5 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 1.0 ng/ml, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 1.0× 106 units/mg.|
MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK
NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI
DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES
|Measured Molecular Weight||26.5 kDa, observed by non-reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against 25mM Tris, pH8.0.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human Interleukin-5 (rhIL-5) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-5 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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