GH, Human

Name: GH, Human
SKU: Z03012-1
Rating: 5 (7 reviews)

Overview

Synonyms	GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin
Description	Growth Hormone (GH) is a member of the somatotropin/prolactin family which play an important role in growth control. The human GH cDNA encodes a 217 amino acid (aa), and the first 26 aa is a signal peptide. By alternative splicing, at least four isoforms of GH have been identified. The major role of GH in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. GH stimulates both the differentiation and proliferation of myoblasts, and also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Recombinant human Growth Hormone (rhGH) produced in E. coli is a single non-glycosylated polypeptide chain containing 191 amino acids. A fully biologically active molecule, rhGH has a molecular mass of 22.1 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Species	Human
Source	E. coli
Biological Activity	ED₅₀ < 0.5 ng/ml, measured by a cell proliferation assay using Nb2-11 Cells, corresponding to a specific activity of > 2.0× 10⁶ units/mg.
Sequence	FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

Measured Molecular Weight	22.1 kDa, observed by reducing SDS-PAGE.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Formulation	Lyophilized after extensive dialysis against PBS.
Reconstitution	Reconstituted in ddH₂O at 100 μg/ml.
Endotoxin Level	< 0.2 EU/μg, determined by LAL method.
Storage	Lyophilized recombinant human Growth Hormone (rhGH) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhGH should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note	For research use only

ED₅₀< 0.5 ng/ml, measured by a cell proliferation assay using Nb2-11 Cells, corresponding to a specific activity of > 2.0× 10⁶ units/mg.