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IL-12, Human(HEK 293-expressed)

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the mouse system.
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Z03023-5

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Product Introduction
Species Human
Protein Construction
IL-12 p35 (Arg23-Ser219)
Accession # P29459
IL-12 p40 (Ile23-Ser328)
Accession # P29460
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 2.0 ng/ml, measured by the induction of IFN-γ from NK cells, corresponding to a specific activity of > 5.0 × 105 U/mg.
Expression System HEK 293
Theoretical Molecular Weight 75 kDa
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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IL-12, Human(HEK 293-expressed)

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IL-12, Human(HEK 293-expressed)

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IL-12, Human(HEK 293-expressed)

ED₅₀< 2 ng/ml, measured by the induction of IFN-γ from NK cells co-stimulated with IL-2, corresponding to a specific activity of > 5×10⁵ units/mg. »

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Background
Target Background Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the mouse system.
Synonyms IL12; NKSF; TCMF; CLMF; TSF; CTL maturation factor; Cytotoxic lymphocyte maturation factor
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.