• English
  • Sign In
  • Contact Us
Catalog Products » Recombinant Proteins » Enzymes and Inhibitors » Tobacco Etch Virus Protease (TEV Protease), His

TEV Protease, His

Synonyms rTEV, TEV, P1 protease
Description Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Source E. coli
Biological Activity 10 IU/μl. Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.

Measured Molecular Weight 28.4 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analyses.
Formulation Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5.
Storage Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.
Note For research use only

Notice there is no results found, you can visit "Citations Database" for other results.

For more documents, please visit "Technical Support".

Cat. No. Z03030
1000 IU $39.00
10000 IU $210.00