|Synonyms||kallikrein-related peptidase 11, TLSP, hippostasin, Kallikrein-11, hK11, Hippostasin, Trypsin-like protease, Serine protease 20, PRSS20, MGC33060, EC 3.4.21.|
|Description||Kallikreins are a subgroup of serine proteases having diverse physiological functions. Kallikrein-11 (KLK-11) is possible multifunctional protease.KLK11 efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Recombinant human Kallikrein-11(rhKLK-11) secreted in Sf9 insect cells is a single glycosylated polypeptide chain containing 232 amino acids. A fully biologically active molecule, rhKallikrein-11 has a molecular mass of 35.0 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Source||Sf9 insect cells|
|Biological Activity||KLK-11 specific activity is > 2000 pmole/min/μg when measured by 100uM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester).|
EFAATMLLVN QSHQGFNKEH TSKMVSAIVL YVLLAAAAHS
AFAHHHHHHG SGSDDDDKET RIIKGFECKP HSQPWQAALF
EKTRLLCGAT LIAPRWLLTA AHCLKPRYIV HLGQHNLQKE
EGCEQTRTAT ESFPHPGFNN SLPNKDHRND IMLVKMASPV
SITWAVRPLT LSSRCVTAGT SCLISGWGST SSPQLRLPHT
LRCANITIIE HQKCENAYPG NITDTMVCAS VQEGGKDSCQ
GDSGGPLVCN QSLQGIISWG QDPCAITRKP GVYTKVCKYV
|Measured Molecular Weight||35.0 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS, pH7.4|
|Reconstitution||Reconstituted in ddH2O at 100μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Kallikrein-11(rhKLK-11) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhKLK-11 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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