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Catalog Products » Recombinant Proteins » Growth Factors » Fibroblast Growth Factor-10 (FGF-10), His, Human

FGF-10, His, Human

*This product has been discontinued!*
Synonyms Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2
Description Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares the homolog and receptor FGFR2-IIIb with KGF. However, unlike KGF which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during the multi-organ development via the instructive mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development, and is regulated by Shh during early development.
Recombinant human Fibroblast Growth Factor-10 (rhFGF-10) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 21.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No O15520
Species Human
Source E. coli
Biological Activity ED50 < 20 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0× 104 units/mg.
Sequence
MNHKVHHHHH HMDDDDKMLG QDMVSPEATN SSSSSFSSPS
SAGRHVRSYN HLQGDVRWRK LFSFTKYFLK IEKNGKVSGT
KKENCPYSIL EITSVEIGVV AVKAINSNYY LAMNKKGKLY
GSKEFNNDCK LKERIEENGY NTYASFNWQH NGRQMYVALN
GKGAPRRGQK TRRKNTSAHF LPMVVHS

Measured Molecular Weight 21.4 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Fibroblast Growth Factor-10 (rhFGF-10) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-10 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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