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Catalog Products » Recombinant Proteins » Growth Factors » Fibroblast Growth Factor-acidic (FGF-acidic), Mouse

FGF-acidic, Mouse

Synonyms Fibroblast Growth Factor-acidic, FGF-1, HBGF-1, ECGF-beta
Description Fibroblast Growth Factor- acidic (FGF-acidic) is a mitogen targeting at the endothelial cells, and belongs to the heparin binding FGF family, which contains 22 members. FGF-acidic binds to the receptor family FGFR1-4 in vivo with the assistance of heparin. However, along with FGF -basic, FGF-acidic lacks the signal peptide segment, and thus is not secreted via endoplasmic reticulum (ER) and Golgi bodies. Studies have shown that FGF-acidic is highly regulated, and it is a direct angiogenesis factor. If unregulated, angiogenesis could contribute to several diseases including arthritis, diabetes, ocular neovascularization, and especially tumors. Therefore, FGF-acidic is treated as a potential oncogene, and its overexpression is correlated tightly with several cancers.
Recombinant mouse Fibroblast Growth Factor- acidic (rmFGF-acidic) produced in E. coli is a single non-glycosylated polypeptide chain containing 140 amino acids. A fully biologically active molecule, rmFGF-acidic has a molecular mass of 15.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P61148
Species Mouse
Source E. coli
Biological Activity ED50 < 0.4 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 10 µg/mL heparin, corresponding to a specific activity of > 2.5× 106 units/mg.
Sequence
FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ
HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN
EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG
PRTHYGQKAI LFLPLPVSSD

Measured Molecular Weight 15.8 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse Fibroblast Growth Factor- acidic (rmFGF-acidic) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmFGF-acidic should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • FGF-acidic, Mouse
  • FGF-acidic, Mouse

    ED₅₀<0.4 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 10 µg/mL heparin, corresponding to a specific activity of > 2.5× 10⁶ units/mg.




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Cat. No. Z03049
Size
Price
10 ug $40.00
50 ug $85.00
1 mg $625.00