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Catalog Products » Recombinant Proteins » Growth Factors » Sonic Hedgehog (C25II) (Shh), Mouse

Shh (C25II), Mouse

Synonyms Hhg1
Description Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.
Recombinant mouse Sonic Hedgehog (C25II) (rmShh) produced in E. coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rmShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No Q62226
Species Mouse
Source E. coli
Biological Activity ED50 < 2.0 μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500 units/mg.
Sequence
IIGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY
EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC
KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE
GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH
CSVKAENSVA AKSGG

Measured Molecular Weight 19.7 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse Sonic Hedgehog (C25II) (rmShh) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmShh should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • Shh (C25II), Mouse
  • Shh (C25II), Mouse

    ED₅₀<2.0 μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500 units/mg.




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Cat. No. Z03050
Size
Price
10 ug $65.00
50 ug $159.00
1 mg $1200.00