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 Z03061.jpg&file=scm_files/productFile_notes/2015/04/26/5aa008d2e0d82e7b2b5d8027f3fb1198.jpg)
| Z03061 | |
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| Ask us a question | |
| Species | Human |
| Amino Acid Sequence |
00001 SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII 00041 VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS |
| Purity | > 95% as analyzed by SDS-PAGE and HPLC |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Biological Activity | ED50 < 20 ng/mL, measured by the FLIPR assay using CHO cells transfected with human CXCR1, the receptor of human CXCL8, corresponding to a specific activity of > 5×104 units/mg. |
| Expression System | E. coli |
| Apparent Molecular Weight | 8.4 kDa, observed by reducing SDS-PAGE |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O at 100 μg/mL |
| Storage & Stability | Lyophilized recombinant human Interleukin-8/CXCL8 (rhIL-8) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-8 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
2 μg of IL-8(aa8-79), Human (Cat. No. Z03061) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining. »
| Target Background |
Interleukin-8 is one of the first discovered
chemokines, and belongs to the CXCL family, in
which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two
forms: 77 a.a. produced by endothelial cells, and
the more active 72 a.a. produced by monocytes.
The receptors of IL-8 are the seven-helical G-protein
coupled receptors CXCR1 and CXCR2, exclusively
expressed on neutrophils. The functions of IL-8 are
to induce rapid changes in cellular shape, activate
the integrins, and release the granule contents of
neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells. Recombinant human I Interleukin-8/CXCL8 (rhIL- 8) produced in E. coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhIL-8 has a molecular mass of 8.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Synonyms | CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF), NAP-1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
