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VEGF165, Human(HEK 293-expressed)

Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
$100.00
Z03073-10

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Product Introduction
Species Human
Protein Construction
VEGF165 (Ala27-Arg191)
Accession # P15692-4
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 5.0 ng/ml, measured in a cell proliferation assay using HUVEC cells.
Expression System HEK 293
Apparent Molecular Weight 20~26 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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VEGF165, Human(HEK 293-expressed)

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VEGF165, Human(HEK 293-expressed)

»

VEGF165, Human(HEK 293-expressed)

ED₅₀< 5 ng/ml, measured in a cell proliferation assay using HUVEC cells. »

VEGF165, Human(HEK 293-expressed)

GenScript product showed better activity compared to competitor P »

VEGF165, Human(HEK 293-expressed)

GenScript product showed better activity compared to competitor R »

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Background
Target Background Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
Synonyms VEGF-165; Vascular Endothelial Growth Factor 165; VPF; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen; Vascular Permeability Factor
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Antonín Sedlář, et al. Growth Factors VEGF-A and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation. Int J Mol Sci. (2021-02)