|Synonyms||Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, Fgf8|
|Description||Fibroblast Growth Factor 8c (FGF-8c) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. In different species, e.g. human and mouse, FGF-8 has 8 different isoforms, from FGF-8a to FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, thus conduct different signaling cascade pathways. FGF-8 has very widespread expression pattern during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression; on the other hand, FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation.
Recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) produced in E. coli is a single non-glycosylated polypeptide chain containing 247 amino acids. A fully biologically active molecule, rmFGF-8c has a molecular mass of 28.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 150 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 6.7× 103 units/mg.|
MQVRSAAQKR GPGAGNPADT LGQGHEDRPF GQRSRAGKNF
TNPAPNYPEE GSKEQRDSVL PKVTQRHVRE QSLVTDQLSR
RLIRTYQLYS RTSGKHVQVL ANKRINAMAE DGDPFAKLIV
ETDTFGSRVR VRGAETGLYI CMNKKGKLIA KSNGKGKDCV
FTEIVLENNY TALQNAKYEG WYMAFTRKGR PRKGSKTRQH
QREVHFMKRL PRGHHTTEQS LRFEFLNYPP FTRSLRGSQR
|Measured Molecular Weight||28.2 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmFGF-8c should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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