• English
  • Sign In
  • Contact Us
×
Catalog Products » Recombinant Proteins » Cytokines » gAcrp30/Adipolean, Human

gAcrp30/Adipolean, Human

Synonyms apm-1, Adipolean (human)
Description gAcrp30/Adipolean is the globular head domain of Adipocyte complement-related protein of 30 kDa (Acrp30), a cytokine expressed in adipocytes. The name of Acrp30 is bases on its closest homolog, complement factor c1q, and the globular domain of Acrp30 has an unexpected homolog with the Tumor Necrosis Factor (TNF) family of cytokines. Acrp30 is the isoform of adiponectin, and shares the two receptors with adiponectin: adipoR1 expressed in skeletal muscle, and adipoR2 expressed in liver. The expression level of Acrp30 in adipocytes is negatively correlated with body weight, and is lower in obese mouse than normal mouse. The globular domain of Acrp30 induces free fatty acid oxidation in muscle and weight reduction in mouse, therefore shows potential pharmacological effects in obesity.
Recombinant human gAcrp30/Adipolean (rhgAcrp30) produced in E. coli is a single non-glycosylated polypeptide chain containing 144 amino acids. A fully biologically active molecule, rhgAcrp30 has a molecular mass of 16.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No Q15848
Species Human
Source E. coli
Biological Activity ED50 < 10 ug/mL, measured by a cell growth inhibition assay using M1 cells, corresponding to a specific activity of > 100 units/mg.
Sequence
KGEPGEGAYV YRSAFSVGLE TYVTIPNMPI RFTKIFYNQQ
NHYDGSTGKF HCNIPGLYYF AYHITVYMKD VKVSLFKKDK
AMLFTYDQYQ ENNVDQASGS VLLHLEVGDQ VWLQVYGEGE
RNGLYADNDN DSTFTGFLLY HDTN

Measured Molecular Weight 16.6 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human gAcrp30/Adipolean (rhgAcrp30) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhgAcrp30 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. Z03091
Size
Price
50 ug $159.00
10 ug $65.00
1 mg $1500.00